ABSTRACT
Objective To investigate the changes of hypoxia-inducible factor (HIF-1α, HIF-2α) expression level in lung cancer A-549 cells under normoxic conditions, different hypoxia durations, and different oxygen concentrations. Methods A549 cells were divided into normoxic group, time control group, and oxygen concentration control group. Western blot was used to detect the expression of HIF-1α and HIF-2α in A-549 cells.Results The expression of HIF-1α and HIF-2α protein were lower under normoxia and significantly increased under hypoxic conditions. The difference was statistically significant. The lower the oxygen concentration, the more HIF-1α and HIF-2α protein expression levels were. The differences between high and high were statistically significant. The expression of HIF-1α protein increased at 2 h after hypoxia, peaked at 8 h, appeared plateau at 8 to 16 h, and decreased at 32 h, with a statistically significant difference. HIF-2α proteins gradually increased with prolonged hypoxia. Conclusions Under hypoxic conditions, the expression of HIF-1α and HIF-2α are increased, and the expression of HIF-2α has a time-dependent pattern, which may have more important biological significance.
ABSTRACT
Objective To investigate the intervention of low molecular weight heparin on lung function and coagulation function of patients experiencing acute exacerbation of chronic obstructive pulmonary disease (COPD).Methods Senventy patients,hospitalized in our department from 2009 -2010,with acute exacerbation of COPD,were randomly divided into control group ( 32 patients) and anticoagulation group ( 38 patients).The control group was treated with oxygen inhalation,antibiotics,antispasmodic asthma,relieving cough and phlegm.The anticoagulation group received low molecular weight heparin 4100 IU AXa/times by abdominal subcutaneous injection at 12 h intervals addition to that of control group.Ten days were considered as a course of treatment in both groups.All patients received pulmonary function and blood coagulation testing before and after treatment.Results After treatment,lung function and arterial blood gas indices were significantly improved in both groups ( the anticoagulation group:t =8.2064,6.9207,9.4351,7.5845,7.5241 ; the control group t =5.5588,3.0795,3.9374,2.8488,2.7726,all P ＜ 0.01 ),but the patients in anticoagulation group were improved more significantly than the control group( t =3.2732 4.0167,7.3392,2.2528,3.0008,Pall ＜ 0.01 ) ;anticoagulation treatment greatly improved the plasma D-dimer ( D-dimer,DD) content and the coagulation parameters in anticoagulation group ( comparing with that before treatment,t =5.721,23.235,25.318,23.841,11.354,Pall ＜ 0.01 ; comparing with that of control,t =7.738,19.365,23.373,21.008,9.712,Pall ＜ 0.01 ),while the control group did not produce significant improvement after treatment (t =1.9409,8.0000,1.1592,1.6562,1.2726,P all ＞ 0.05 ).There were had no serious adverse reactions in the anticoagulation treatment group.Conclusion Although the conventional treatment can improve the symptoms of the patients with COPD,but it can't effectively improve the patient's blood hypercoagulability.Conventional therapy combined with low molecular weight heparin anticoagulant therapy is able to improve the blood coagulation hence the lung function in COPD patients.